Human Polθ Polymerase creates DNA from RNA, mRNA vaccines
Polymerases were thought to only work in one direction DNA into DNA or RNA but a new study shows that segments of RNA can and are rewritten into new DNA strands. This contradicts mainstream thought in biology and could have wider implications on other fields science. This also categorically debunks mainstream opinion that mrna-based vaccines Pfizer and Moderna can’t affect the DNA strands of those inoculated.
“This work opens the door to many other studies that will help us understand the significance of having a mechanism for converting RNA messages into DNA in our own cells,” says the lead researcher Pomerantz of Thomas Jefferson University. “The reality that a human polymerase can do this with high efficiency, raises many questions.”
Mammals have 14 DNA polymerases three of which can duplicate the entire genome to prepare for cell division. Eleven others are used to detect and repair DNA strands that break or have errors. Polymerase theta is one of those but is very inefficient in that the polymerase itself is error-prone. The authors of the study noticed that some of polymerase theta’s bad qualities are similar to those known to exist with reverse transcriptase (specifically that of viruses).
Like Pol theta, HIV reverse transcriptase acts as a DNA polymerase, but can also bind RNA and turn it into a DNA strand. Researchers tested polymerase theta against the reverse transcriptase from HIV and found that polymerase theta was capable of converting RNA messages into DNA, which it did as well as HIV reverse transcriptase. Polymerase theta was more efficient and introduced fewer errors when using an RNA template to write new DNA messages, than when duplicating DNA into DNA, suggesting that this function could be its primary purpose in the cell.
The group used x-ray crystallography to define the structure and found that polymerase theta was able to change shape in order to accommodate different sizes of RNA molecules which no other polymerases have the capacity to do.
“Our research suggests that polymerase theta’s main function is to act as a reverse transcriptase,” “In healthy cells, the purpose of this molecule may be toward RNA-mediated DNA repair. In unhealthy cells, such as cancer cells, polymerase theta is highly expressed and promotes cancer cell growth and drug resistance. It will be exciting to further understand how polymerase theta’s activity on RNA contributes to DNA repair and cancer-cell proliferation.”Dr. Pomerantz.